Anti-gliadin IgA and IgG kits

• Info leaflet (PDF, 576 KB)
• Kit insert (PDF, 687 KB)
• User's guide (PDF, 110 KB)

Contents

• 96 wells in microtiter strips
• positive reference and negative control
• all components in ready to use form

Properties

• all reactions at room temperature
• hands on -time 30-45 minutes
• total assay time 3 hours
• shelf life 1 year at +4°C

Performance characteristics

Interpretation of results for gliadin IgA and IgG antibodies with age dependent reference limits as guidelines
Sensitivity and specificity of IgA and IgG antigliadin ELISA (> 2 years of age)

Labmaster ELISA kits for IgA and IgG antibodies give quantitative results. Results are expressed as arbitrary units (AU) as percentage of the positive reference.

Reference limits have been established for two age groups separately. The borderline between the age groups is at two years of age. By comparing the results for a patient sample with cut off limits for the proper age group, the diagnostic specificity and sensitivity is optimized. The results can be used as an aid in the clinical diagnosis of coeliac disease.

New enzyme label: Inorganic pyrophosphatase

• sensitive enzyme label
• stable enzyme and enzyme conjugates
• noncarcinogenic colour reagents
• room temperature reactions
• clearly visible colour reaction from yellow to green
• measurement with microtiter plate reading photometer at 620 nm

Colour reaction principle

One inorganic pyrophosphatase molecule cleaves 6 pyrophosphates to 12 phosphate molecules.

Ordering

See Ordering information.

References