Labmaster Daidzein TR-FIA

What is daidzein—Background

Isoflavonoids are a group of diphenolic hormone-like compounds of dietary origin that are of great interest particularly because of their anti-carcinogenic potency, but also because of their association with other Western diseases like coronary heart disease.

The main phytoestrogens derived from the diet are genistein, daidzein and glycitein. They nearly merely occur as glycosidic conjugate forms in most soy-protein products in high concentrations.

When consumed, isoflavonoids are converted by the intestinal microflora to biologically active secondary plant metabolites. Ingested glycosides are rapidly hydrolyzed by the gut bacteria, whereafter the aglycones undergo further metabolism. In this way the aglycone daidzein, which is formed from formononetin, is reduced by the intestinal microflora to the isoflavan equol (about 70 %) and O-desmethyl- angolensin (about 5-20 %), but this process happens to a substantial extent in only 30% of people.

Figure of Daidzein molecule

Quantitative time-resolved fluoroimmunoassay

Method—Competitive immunoassay

• A sample preparation by hydrolyse and ether extraction

Chart of Labmaster Daidzein TR-FIA method, Figure 1

• 90 minutes incubation

• Aspiration and washing

Chart of Labmaster Daidzein TR-FIA method, Figure 2

• 5 minutes incubation

Chart of Labmaster Daidzein TR-FIA method, Figure 3

• Fluorescence measurement


Ordering information

Labmaster Daidzein TR-FIA

For local representatives in Europe, see Ordering information.

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