Labmaster Enterolactone TR-FIA

Background
Lignans are a group of diphenolic hormone-like compounds of dietary origin that are of great interest particularly because of their anti-carcinogenic potency, but also because of their association with other Western diseases like coronary heart disease. Lignans occur mainly in various whole-grain cereals (barley, rye and wheat), seeds, nuts, legumes and vegetables.

When consumed, compounds that occur mainly in the glycosidic form in the plants are converted to the mammalian lignans by the intestinal microflora. Ingested glycosides are hydrolysed in the proximal colon. The colonic microflora convert matairesinol to enterolactone and secoisolarici-resinol to enterodiol, and latter is readily oxidised to enterolactone.

Figure of Enterolactone molecule

Enterolactone was identified in the urine of the female vervet monkey and women about twenty years ago. Recent experiments suggest that enterolactone may help prevent the development of cancer as well as atheoclerosis.

Quantitative time-resolved fluoroimmunoassay

Method

• A sample preparation by hydrolyse and ether extraction

Chart of Labmaster Enterolactone TR-FIA method, Figure 1

• 90 minutes incubation

• Aspiration and washing

Chart of Labmaster Enterolactone TR-FIA method, Figure 2

• 5 minutes incubation

Chart of Labmaster Enterolactone TR-FIA method, Figure 3

• Fluorescence measurement


Ordering information

Labmaster Enterolactone TR-FIA

For local representatives in Europe, see Ordering information.

References

  1. Adlercreutz H. Phytoestrogens: Epidemiogy and a possible Role in Cancer Protection. Environmental Health Perspectives 1995;103(Suppl 7):103-12.
  2. Adlercreutz H. and Mazur W. Phyto-oestrogens and Western Diseases. Ann Med 1997;29:95-120.
  3. Adlercreutz H., Guojie J., et al. Time-Resolved Fluoroimmunoassay for Plasma Enterolactone. Analytical Biochemistry 1998;265:208-15.
  4. Adlercreutz H. Phytoestrogens. State of the art. Environmental Toxicology and Pharmalogy 1999;7:201-7.
  5. Adlercreutz H., Lapcík O., et al. Immunoassay of Phytoestrogens in Human Plasma. Journal of Medical Food 1999;2:131-33.
  6. Mazur W., Wähälä K., et al. Dietary Phytoestrogens — From Chemistry to Chemoprevention. Kemia-Kemi 1998;25:48-55.
  7. Pietinen P., Stumpf K., et al. Serum Enterolactone and Risk of Breast Cancer: A case-Control Study in Eastern Finland. Cancer Epidemiology, Bomarkers & Prevention 2001;10:339-44.
  8. Stumpf K., Uehara M., et al. Changes in the Time-Resolved Fluoroimmunoassay of Plasma Enterolactone. Analytical Biochemistry 2000;284:153-57.
  9. Vanharanta M., Voutilainen S., et al. Risk of acute coronary events according to serum concentrations of enterolactone: a prospective population-based case-control study. Lancet 1999; 354:2112-15.